Human GFR alpha-like Protein

• Catalog Number: orb1146970
• Category: Proteins
• Description: GFR alpha-like is also known as GDNF family receptor alpha-like, GFRAL, C6orf144, UNQ9356, PRO34128. Growth differentiation factor-15 (GDF15) is a circulating protein that has been implicated in multiple biological processes, including energy homeostasis, body weight regulation, and cachexia driven by cancer and chronic disease. GDNF family receptor α-like (GFRAL) was recently identified as the neuronal brainstem receptor responsible for mediating the anorectic actions of GDF15. Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses . Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 64.2 kDa
• Target: GFR alpha-like
• Source: Human GFR alpha-like, Fc Tag (orb1146970) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Glu 351 (Accession # Q6UXV0-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human GDF-15, His Tag at 1 μg/mL (100 μL/well) can bind Human GFR alpha-like, Fc Tag with a linear range of 0.4-25 ng/mL (QC tested).
• Expression Region: Ser 19 - Glu 351
• Endotoxins: 1.0 EU per μg
• NCBI: NP_997293.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
• Alternative names: GFR alpha like,GFR alpha-like,GFRAL,GRAL
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 64.2 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human GDF-15, His Tag (Cat. No. orb624215) at 1 μg/mL (100 μL/well) can bind Human GFR alpha-like, Fc Tag (Cat. No. orb1146970) with a linear range of 0.4-25 ng/mL (QC tested).