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Catalog Number | orb391785 |
---|---|
Category | Proteins |
Description | Recombinant human GCDH protein is produced by our E. coli expression system and the target gene encoding Arg45-Lys438 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM HEPES, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | GCDH |
Entrez | 2639 |
UniProt ID | Q92947 |
Protein Sequence | MNHKVHHHHH HMRPEFDWQD PLVLEEQLTT DEILIRDTFR TYCQERLMPR ILLANRNEVF HREIISEMGE LGVLGPTIKG YGCAGVSSVA YGLLARELER VDSGYRSAMS VQSSLVMHPI YAYGSEEQRQ KYLPQLAKGE LLGCFGLTEP NSGSDPSSME TRAHYNSSNK SYTLNGTKTW ITNSPMADLF VVWARCEDGC IRGFLLEKGM RGLSAPRIQG KFSLRASATG MIIMDGVEVP EENVLPGASS LGGPFGCLNN ARYGIAWGVL GASEFCLHTA RQYALDRMQF GVPLARNQLI QKKLADMLTE ITLGLHACLQ LGRLKDQDKA APEMVSLLKR NNCGKALDIA RQARDMLGGN GISDEYHVIR HAMNLEAVNT YEGTHDIHAL ILGRAITGIQ AFTASK |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM HEPES, 150mM NaCl, pH 7.4. |
Alternative names | Glutaryl-CoA dehydrogenase mitochondrial; GCD Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
≥90% as determined by SDS-PAGE | |
This protein contains the human GCDH(Thr31-Pro204) was fused without Tag and expressed in E. coli. |
≥90% as determined by SDS-PAGE | |
This protein contains the human GCDH(Arg45-Lys438) was fused with the N-terminal His Tag and expressed in E. coli. |
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