Human GCA protein

SKU: orb391783

Description

Recombinant human GCA protein is produced by our E.coli expression system and the target gene encoding Met1-Ile217 is expressed with a GST tag at the N-terminus.

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Reactivity: Human
• Protein Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMAYP GYGGGFGNFS IQVPGMQMGQ PVPETGPAIL LDGYSGPAYS DTYSSAGDSV YTYFSAVAGQ DGEVDAEELQ RCLTQSGING TYSPFSLETC RIMIAMLDRD HTGKMGFNAF KELWAALNAW KENFMTVDQD GSGTVEHHEL RQAIGLMGYR LSPQTLTTIV KRYSKNGRIF FDDYVACCVK LRALTDFFRK RDHLQQGSAN FIYDDFLQGT MAI
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 500mM NaCl, pH 8.5.
• Disclaimer: For research use only

Alternative Names

GCL; Grancalcin

Quick Database Links

UniProt

P28676

NCBI Gene ID

25801