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Catalog Number | orb391773 |
---|---|
Category | Proteins |
Description | Recombinant human GALE protein is produced by our E. coli expression system and the target gene encoding Met1-Ala348 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 50mM Tris-HCl, 150mM NaCl, 2mM DTT, 1mM EDTA, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | GALE |
Entrez | 2582 |
UniProt ID | Q14376 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MAEKVLVTGG AGYIGSHTVL ELLEAGYLPV VIDNFHNAFR GGGSLPESLR RVQELTGRSV EFEEMDILDQ GALQRLFKKY SFMAVIHFAG LKAVGESVQK PLDYYRVNLT GTIQLLEIMK AHGVKNLVFS SSATVYGNPQ YLPLDEAHPT GGCTNPYGKS KFFIEEMIRD LCQADKTWNA VLLRYFNPTG AHASGCIGED PQGIPNNLMP YVSQVAIGRR EALNVFGNDY DTEDGTGVRD YIHVVDLAKG HIAALRKLKE QCGCRIYNLG TGTGYSVLQM VQAMEKASGK KIPYKVVARR EGDVAACYAN PSLAQEELGW TAALGLDRMC EDLWRWQKQN PSGFGTQA |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 50mM Tris-HCl, 150mM NaCl, 2mM DTT, 1mM EDTA, pH 8.0. |
Alternative names | UDP-glucose 4-epimerase; Galactowaldenase; UDP-gal Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
54.3 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human GALE(Met1-Ala348) was fused with the N-terminal His Tag and expressed in E. coli. |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
38.2 kDa | |
E.Coli |
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