Human GABARAP protein

SKU: orb391767

Description

Recombinant human GABARAP protein is produced by our E.coli expression system and the target gene encoding Met1-Lys117 is expressed with a GST tag at the N-terminus.

Research Area

Autophagic, Cancer, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Reactivity: Human
• Protein Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMKFV YKEEHPFEKR RSEGEKIRKK YPDRVPVIVE KAPKARIGDL DKKKYLVPSD LTVGQFYFLI RKRIHLRAED ALFFFVNNVI PPTSATMGQL YQEHHEEDFF LYIAYSDESV YGL
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μM filtered solution of 50mM Tris-HCl, 200mM NaCl, pH 7.5.
• Disclaimer: For research use only

Alternative Names

GABA(A) receptor-associated protein

Quick Database Links

UniProt

Q6IAW1

NCBI Gene ID

11337