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Human Flt3 Ligand (Sf9) Protein
Catalog Number: orb425560
| Catalog Number | orb425560 |
|---|---|
| Category | Proteins |
| Description | Recombinant of human Flt3 Ligand (Sf9) protein |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser |
| Source | Baculovirus Sf9 cells |
| Biological Activity | The ED50, calculated by the dose-dependent stimulation of the proliferation of human MUTZ-2 cells is < 3.0 ng/ml. |
| Storage | Stability: Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-L should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
| Buffer/Preservatives | The protein was lyophilized with no additives. |
| Alternative names | Fms-related tyrosine kinase 3 ligand, FLK2, STK1, Read more... |
| Note | For research use only |
| Application notes | Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.42 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard of Flt3-Ligand as a Reference Standard |
| Expiration Date | 6 months from date of receipt. |
