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Catalog Number | orb81121 |
---|---|
Category | Proteins |
Description | Human Fibronectin protein |
Form/Appearance | Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | The Fibronectin was lyophilized from a non sterile 2mg/ml buffer of 10mM sodium phosphate, pH 7.5 and 0.15M NaCl. |
Purity | ≥ 95.0% as determined by SDS-PAGE. |
Application notes | Recombinant & Natural Proteins |
Source | Human Plasma |
Solubility (25°C) | We suggest reconstituting the 1mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2mg/ml using sterile water. Let stand 1-2 hours. The recommended concentration is 4M-5M urea. When using the protein as an attachment factor, wash the urea off after attaching the fibronectin to the growth surface (plate or dish). |
Storage | Stability: Store the lyophilized fibronectin at 4°C. Upon reconstitution fibronectin should be stored at 4°C for 2 weeks and for future use below -18°C. Please prevent freeze-thaw cycles |
Note | For research use only |
Greater than 85% as determined by reducing SDS-PAGE. | |
49.6 KDa | |
Mammalian |