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Catalog Number | orb80806 |
---|---|
Category | Proteins |
Description | Recombinant of Human FGFR4 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | CD334 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. |
Purity | Greater than 90.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Application notes | Protein Kinases |
Source | Insect Cells |
Biological Activity | Determined by its ability to inhibit human FGF acidic-dependent proliferation on R1 cells. The ED50 for this effect is typically at 15.0-30.0 ng/ml. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized FGFR-4 in sterile PBS not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized FGFR4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGFR4 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Alternative names | Fibroblast Growth Factor Receptor 4, EC 2.7.10.1, Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 39.3 kDa after removal of the signal peptide.The apparent molecular mass of FGFR4-His is approximately 35-70 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result. |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 80-95 kDa based on Tris-Bis PAGE result. |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 55-72 kDa based on Tris-Bis PAGE result. |