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Catalog Number | orb594758 |
---|---|
Category | Proteins |
Description | Recombinant Human Fibroblast growth factor 8(FGF8),partial (Active) |
Reactivity | Human |
Tag | N-terminal 6xHis-tagged |
Form/Appearance | Lyophilized powder |
Purity | Greater than 95% as determined by SDS-PAGE. |
MW | 27.7 kDa |
UniProt ID | P55075 |
Protein Sequence | QEGPGRGPALGRELASLFRAGREPQGVSQQVTVQSSPNFTQHVREQSLVTDQLSRRLIRTYQLYSRTSGKHVQVLANKRINAMAEDGDPFAKLIVETDTFGSRVRVRGAETGLYICMNKKGKLIAKSNGKGKDCVFTEIVLENNYTALQNAKYEGWYMAFTRKGRPRKGSKTRQHQREVHFMKRLPRGHHTTEQSLRFEFLNYPPFTRSLRGSQRTWAPEPR |
Protein Length | Partial of Isoform 4 |
Source | E.coli |
Biological Activity | The ED50 as determined in a cell proliferation assay using BALB/c 3T3 cells is less than 0.5 ug/ml. |
Expression Region | 23-244aa |
Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.4 |
Alternative names | Fibroblast growth factor 8;Androgen-induced growth Read more... |
Note | For research use only |
Application notes | Partial of Isoform 4 |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
HPLC, SDS-PAGE | |
Unconjugated | |
> 97% by SDS-PAGE and HPLC analyses | |
28.1kDa |
Greater than 95% as determined by SDS-PAGE. | |
22.5 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human FGF8(Gln23-Arg215) was fused without Tag and expressed in E. coli. |
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