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Catalog Number | orb49385 |
---|---|
Category | Proteins |
Description | Recombinant human FGF21 protein |
Tag | N-His |
Form/Appearance | Lyophilized from a 0.2 um filtered solution of 20mM Tris-HCl, 100mM NaCl, 2mM EDTA, pH 9.0. |
UniProt ID | AAH18404.1 |
Protein Sequence | His29-Ser209 |
Source | E.coli |
Expression System | E. coli |
Endotoxins | Less than 0.1 ng/ug (1 EU/ug) |
Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 100mM NaCl, 2mM EDTA, pH 9.0 . |
Alternative names | Fibroblast Growth Factor 21 protein, FGF21 protein Read more... |
Note | For research use only |
Application notes | 6His tag at the N-terminus. |
Expiration Date | 6 months from date of receipt. |
> 96% as determined by SDS-PAGE and HPLC. | |
19.4 kDa | |
E.Coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
HPLC, SDS-PAGE | |
Unconjugated | |
> 97% by SDS-PAGE and HPLC analyses | |
19.9 kDa |
SDS-PAGE | |
Unconjugated | |
> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.5 kDa after removal of the signal peptide. The apparent molecular mass of FGF21-hFc is approximately 40-55 kDa due to glycosylation. | |
Mammalian |
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