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Catalog Number | orb391731 |
---|---|
Category | Proteins |
Description | Recombinant human FGF21 protein is produced by our E.coli expression system and the target gene encoding His29-Ser209 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 200mM NaCl, 1mM EDTA, 1mM DTT, pH 7.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | FGF21 |
Entrez | 26291 |
UniProt ID | Q9NSA1 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPAPPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 200mM NaCl, 1mM EDTA, 1mM DTT, pH 7.0. |
Alternative names | Fibroblast growth factor 21; FGF-21 Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 96% as determined by SDS-PAGE and HPLC. | |
19.4 kDa | |
E.Coli |
HPLC, SDS-PAGE | |
Unconjugated | |
> 97% by SDS-PAGE and HPLC analyses | |
19.9 kDa |
SDS-PAGE | |
Unconjugated | |
> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.5 kDa after removal of the signal peptide. The apparent molecular mass of FGF21-hFc is approximately 40-55 kDa due to glycosylation. | |
Mammalian |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
KMP1956, Recombinant Human FGF21 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (His Tag29-Ser210) of Human FGF21 fused with a mFc and Avi tag at the N-terminal. |
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