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Catalog Number | orb391728 |
---|---|
Category | Proteins |
Description | Recombinant human FGF2 protein is produced by our E. coli expression system and the target gene encoding Met134-Ser288 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | FGF2 |
Entrez | 2247 |
UniProt ID | P09038 |
Protein Sequence | MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5. |
Alternative names | FGFB; Fibroblast growth factor 2; FGF-2; Basic fib Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
16.5 kDa | |
Human FGF basic, premium grade (orb257225) is expressed from E. coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4). |
Greater than 90% as determined by SDS-PAGE. | |
32.4 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
17.9 kDa | |
Yeast |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
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