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Catalog Number | orb80021 |
---|---|
Category | Proteins |
Description | FGF acidic Human Recombinant (FGF-1) produced in E.coli is a single, non-glycosylated, polypeptide chain containing 141amino acids and having a molecular mass of 15967 Dalton.The FGF acidic is purified by proprietary chromatographic techniques. |
Tested applications | SDS-PAGE |
Purity | > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | FGF 1 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic in sterile H2O at degrees Celsius at concentration of 0.1mg-0.2 umg per 1ml. Allow sample to sit for min. at degrees, spin to remove precipitant. |
Protein Sequence | MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D. |
Biological Activity | The ED50, calculated by the dose-dependant proliferation of mouse BALB/c 3T3 cells is < 1.0 ng/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The protein was lyophilized from concentrated (1mg/ml) sterile solution containing 10mM sodium phosphate pH=7.5 and 75mM NaCl. |
Alternative names | HBGF-1 protein, ECGF-beta protein, FIBP protein, F Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Greater than 85% as determined by SDS-PAGE. | |
17.8 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
HPLC, SDS-PAGE | |
Unconjugated | |
> 96% by SDS-PAGE and HPLC analyses | |
15.8 kDa |
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