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Catalog Number | orb425472 |
---|---|
Category | Proteins |
Description | Recombinant of human FGF 19 (His) protein |
Form/Appearance | Filtered white lyophilized powder. |
Buffer/Preservatives | Filtered (0.4µm) and lyophilized from 0.5mg/ml in 20mM TRIS, 20mM NaCl, pH 7.5. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | MRGSHHHHHH GMASLAFSDA GPHVHYGWGD PIRLRHLYTS GPHGLSSCFL RIRADGVVDC ARGQSAHSLLEIKAVALRTV AIKGVHSVRY LCMGADGKMQ GLLQYSEEDC AFEEEIRPDG YNVYRSEKHR LPVSLSSAKQ RQLYKNRGFL PLSHFLPMLP MVPEEPEDLR GHLESDMFSS PLETDSMDPF GLVTGLEAVR SPSFEK |
Application notes | Cytokines And Growth Factors |
Source | Escherichia Coli |
Solubility (25°C) | Add deionized water to a working concentration approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture. |
Storage | Stability: Lyophilized FGF-19 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor-19 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Alternative names | Fibroblast growth factor 19, FGF-19. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 22.3 kDa after removal of the signal peptide. The apparent molecular mass of FGF19-His is approximately 15-25 kDa due to glycosylation. | |
E.coli |
>95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result. |
> 97% by SDS-PAGE. | |
Recombinant human FGF-19 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Phe27-Lys216) of human FGF-19 (Accession #NP_005108.1) fused with a 6��His Tag at the C-terminus. |
≥90% as determined by SDS-PAGE | |
This protein contains the human FGF19(Phe27-Lys216) was fused with the N-terminal His Tag and expressed in E. coli. |
111.6 kDa (predicted). Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result. |