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Catalog Number | orb391694 |
---|---|
Category | Proteins |
Description | Recombinant human F9 protein is produced by our mammalian expression system and the target gene encoding Thr29-Thr461 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | F9 |
Entrez | 2158 |
UniProt ID | P00740 |
Protein Sequence | TVFLDHENAN KILNRPKRYN SGKLEEFVQG NLERECMEEK CSFEEAREVF ENTERTTEFW KQYVDGDQCE SNPCLNGGSC KDDINSYECW CPFGFEGKNC ELDVTCNIKN GRCEQFCKNS ADNKVVCSCT EGYRLAENQK SCEPAVPFPC GRVSVSQTSK LTRAETVFPD VDYVNSTEAE TILDNITQST QSFNDFTRVV GGEDAKPGQF PWQVVLNGKV DAFCGGSIVN EKWIVTAAHC VETGVKITVV AGEHNIEETE HTEQKRNVIR IIPHHNYNAA INKYNHDIAL LELDEPLVLN SYVTPICIAD KEYTNIFLKF GSGYVSGWGR VFHKGRSALV LQYLRVPLVD RATCLRSTKF TIYNNMFCAG FHEGGRDSCQ GDSGGPHVTE VEGTSFLTGI ISWGEECAMK GKYGIYTKVS RYVNWIKEKT KLTVDHHHHH H |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0. |
Alternative names | Coagulation factor IX; Christmas factor; Plasma th Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 85% as determined by SDS-PAGE. | |
42.0 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human F9(Thr29-Thr461) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
Unconjugated | |
95% | |
50.6 kDa | |
Human Coagulation factor IX Protein, His Tag (orb1499257) is expressed from human 293 cells (HEK293). It contains AA Thr 29 - Thr 461 (Accession # P00740-1). |
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