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Human F9 protein

SKU: orb391694

Description

Recombinant human F9 protein is produced by our mammalian expression system and the target gene encoding Thr29-Thr461 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
TargetF9
ReactivityHuman
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceTVFLDHENAN KILNRPKRYN SGKLEEFVQG NLERECMEEK CSFEEAREVF ENTERTTEFW KQYVDGDQCE SNPCLNGGSC KDDINSYECW CPFGFEGKNC ELDVTCNIKN GRCEQFCKNS ADNKVVCSCT EGYRLAENQK SCEPAVPFPC GRVSVSQTSK LTRAETVFPD VDYVNSTEAE TILDNITQST QSFNDFTRVV GGEDAKPGQF PWQVVLNGKV DAFCGGSIVN EKWIVTAAHC VETGVKITVV AGEHNIEETE HTEQKRNVIR IIPHHNYNAA INKYNHDIAL LELDEPLVLN SYVTPICIAD KEYTNIFLKF GSGYVSGWGR VFHKGRSALV LQYLRVPLVD RATCLRSTKF TIYNNMFCAG FHEGGRDSCQ GDSGGPHVTE VEGTSFLTGI ISWGEECAMK GKYGIYTKVS RYVNWIKEKT KLTVDHHHHH H

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Coagulation factor IX; Christmas factor; Plasma thromboplastin component; PTC

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

10 μg
$ 280.00
50 μg
$ 640.00
500 μg
$ 4,190.00
DispatchUsually dispatched within 5-10 working days
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