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Catalog Number | orb391688 |
---|---|
Category | Proteins |
Description | Recombinant human Estrogen Receptor beta protein is produced by our E. coli expression system and the target gene encoding Met1-Ala323 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 50mM Tris-HCl, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | ESR2 |
Entrez | 2100 |
UniProt ID | Q92731 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MDIKNSPSSL NSPSSYNCSQ SILPLEHGSI YIPSSYVDSH HEYPAMTFYS PAVMNYSIPS NVTNLEGGPG RQTTSPNVLW PTPGHLSPLV VHRQLSHLYA EPQKSPWCEA RSLEHTLPVN RETLKRKVSG NRCASPVTGP GSKRDAHFCA VCSDYASGYH YGVWSCEGCK AFFKRSIQGH NDYICPATNQ CTIDKNRRKS CQACRLRKCY EVGMVKCGSR RERCGYRLVR RQRSADEQLH CAGKAKRSGG HAPRVRELLL DALSPEQLVL TLLEAEPPHV LISRPSAPFT EASMMMSLTK LADKELVHMI SWAKKIPGMR GNA |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 50mM Tris-HCl, pH 8.0. |
Alternative names | ESTRB; NR3A2; Estrogen receptor beta; ER-beta; Nuc Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
Greater than 90% as determined by SDS-PAGE. | |
51.9 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
39.8 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
61.1 kDa | |
Yeast |
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