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Human ESAM protein
Catalog Number: orb391684
| Catalog Number | orb391684 |
|---|---|
| Category | Proteins |
| Description | Recombinant human ESAM protein is produced by our mammalian expression system and the target gene encoding Gln30-Ala247 is expressed with a Fc tag at the C-terminus. |
| Target | ESAM |
| Reactivity | Human |
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | QLQLHLPANR LQAVEGGEVV LPAWYTLHGE VSSSQPWEVP FVMWFFKQKE KEDQVLSYIN GVTTSKPGVS LVYSMPSRNL SLRLEGLQEK DSGPYSCSVN VQDKQGKSRG HSIKTLELNV LVPPAPPSCR LQGVPHVGAN VTLSCQSPRS KPAVQYQWDR QLPSFQTFFA PALDVIRGSL SLTNLSSSMA GVYVCKAHNE VGTAQCNVTL EVSTGPGAVD DIEGRMDEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK |
| UniProt ID | Q96AP7 |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Source | Human cells |
| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
| Alternative names | Endothelial cell-selective adhesion molecule |
| Note | For research use only |
| Entrez | 90952 |
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Human ESAM protein [orb244917]
Greater than 90% as determined by SDS-PAGE.
39.8 kDa
E.coli
20 μg, 100 μg, 1 mg Human ESAM protein [orb391683]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μgHuman ESAM Protein, Fc Tag [orb2697321]
> 95%, determined by reduced SDS-PAGE
This protein contains (Met 1-Ala 248) of human ESAM (NP_6204112) precursor was expressed with the fused Fc region of human IgG1 at the C-terminus and expressed from HEK293 Cells.
1 mg, 100 μgHuman ESAM Protein, Fc Tag [orb2701785]
> 92%, determined by SDS-PAGE
The mature form of human ESAM (NP_620411.2) extracellular domain (Met 1-Ala 248) with five amino acids (DDDDK) at the C-terminus was expressed from HEK293 Cells.
100 μg, 50 μgHuman ESAM Protein, His Tag [orb2701786]
> 97%, determined by SDS-PAGE
This protein contains the extracellular domain (Met 1-Ala 248) of human ESAM (NP_620411.2) precursor was expressed from HEK293 Cells.
100 μg, 50 μg
