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Catalog Number | orb80017 |
---|---|
Category | Proteins |
Description | Epigen Recombinant Human produced in E.coli is a single, non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7.9 kDa. Epigen is purified by proprietary chromatographic techniques. |
Tested applications | SDS-PAGE |
Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | EPGN |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Epigen in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA. |
Biological Activity | Determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells. The expected ED50 for this effect is 150-300ng/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | EPGN was lyophilized from 20 mM PBS buffer pH-7.4C ontaining 130mM sodium chloride. |
Alternative names | EPG protein, Epigen protein, PRO9904 protein, ALGV Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human Epigen protein
Approximately 7.9kDa monomeric protein, containing 72 amino acid residues, which comprises the EGF homologous portion of the Epigen precursor. | |
Escherichia coli. |
15~20 kDa, observed by reducing SDS-PAGE. | |
CHO |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
7.8 kDa | |
E.Coli |
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