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Human EIF1B protein

SKU: orb391659

Description

Recombinant human EIF1B protein is produced by our E.coli expression system and the target gene encoding Met1-Phe113 is expressed with a 6His tag at the N-terminus.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
ReactivityHuman
Protein SequenceMGSSHHHHHH SSGLVPRGSH MSTIQNLQSF DPFADATKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQR KNICQFLLEV GIVKEEQLKV HGF
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 500mM NaCl, pH 8.0.
DisclaimerFor research use only

Alternative Names

Eukaryotic translation initiation factor 1b; eIF1b; Protein translation factor SUI1 homolog GC20

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human EIF1B protein (orb391659)

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Available Sizes

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10 μg
$ 350.00
50 μg
$ 910.00
500 μg
$ 3,540.00
DispatchUsually dispatched within 5-10 working days
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