Human EGF Protein, His Tag

• Catalog Number: orb762429
• Category: Proteins
• Description: Human EGF Protein, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).
• Reactivity: Human
• Tag: N-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 8.3 kDa
• Target: EGF
• UniProt ID: P01133
• Protein Sequence: NP_001954.2
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Human EGF Protein, His Tag, premium grade (Cat. No. ) at 2 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. EGR-H5252) with a linear range of 0.2-5 ng/mL (QC tested).
• Expression Region: Asn 971 - Arg 1023
• Endotoxins: 0.1 EU per μg
• NCBI: NP_001954.2
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Buffer/Preservatives: PBS, pH 7.4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human EGF Protein, His Tag, premium grade (Cat. No. orb762429) at 2 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. orb257416) with a linear range of 0.2-5 ng/mL (QC tested).