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Description
Research Area
Neuroscience
Images & Validation
−
| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human cells |
|---|---|
| Target | EFNB1 |
| Reactivity | Human |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | LAKNLEPVSW SSLNPKFLSG KGLVIYPKIG DKLDIICPRA EAGRPYEYYK LYLVRPEQAA ACSTVLDPNV LVTCNRPEQE IRFTIKFQEF SPNYMGLEFK KHHDYYITST SNGSLEGLEN REGGVCRTRT MKIIMKVGQD PNAVTPEQLT TSRPSKEADN TVKMATQAPG SRGSLGDSDG KHETVNQEEK SGPGASGGSS GDPDGVDHHH HHH |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EFL3; EPLG2; LERK2; Ephrin-B1; EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2
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Human EFNB1 Protein, His Tag [orb2321401]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 23.8 kDa after removal of the signal peptide. The apparent molecular mass of EFNB1-His is approximately 25-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg Human EFNB1 Protein [orb611128]
Greater than 90% as determined by SDS-PAGE.
Sf9, Baculovirus cells
2 μg, 10 μg, 1 mg
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
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