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Catalog Number | orb391644 |
---|---|
Category | Proteins |
Description | Recombinant human EFNA3 protein is produced by our Mammalian expression system and the target gene encoding Gln23-Ser211 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | EFNA3 |
Entrez | 1944 |
UniProt ID | P52797 |
Protein Sequence | QGPGGALGNR HAVYWNSSNQ HLRREGYTVQ VNVNDYLDIY CPHYNSSGVG PGAGPGPGGG AEQYVLYMVS RNGYRTCNAS QGFKRWECNR PHAPHSPIKF SEKFQRYSAF SLGYEFHAGH EYYYISTPTH NLHWKCLRMK VFVCCASTSH SGEKPVPTLP QFTMGPNVKI NVLEDFEGEN PQVPKLEKSV DHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | EFL2; EPLG3; LERK3; Ephrin-A3; EFL-2; EHK1 ligand; Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 47.5 kDa after removal of the signal peptide. The apparent molecular mass of EFNA3-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
90% | |
48.4 kDa | |
Human Ephrin-A3, Fc Tag (orb257407) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Ser 213 (Accession # AAI10407). |
≥90% as determined by SDS-PAGE | |
This protein contains the human EFNA3(Gln23-Ser211) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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