Human EDF1 protein

SKU: orb391639

Description

Recombinant human EDF1 protein is produced by our E. coli expression system and the target gene encoding Ala2-Lys148 is expressed with a 6His tag at the C-terminus.

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: EDF1
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: AESDWDTVTV LRKKGPTAAQ AKSKQAILAA QRRGEDVETS KKWAAGQNKQ HSITKNTAKL DRETEELHHD RVTLEVGKVI QQGRQSKGLT QKDLATKINE KPQVIADYES GRAIPNNQVL GKIERAIGLK LRGKDIGKPI EKGPRAKLEH HHHHH

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Endothelial differentiation-related factor 1; EDF-1; Multiprotein-bridging factor 1; MBF1

Quick Database Links

Gene Symbol

EDF1

UniProt

O60869

NCBI Gene ID

8721