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Human ECP protein

SKU: orb392392

Description

Recombinant human ECP protein is produced by our Mammalian expression system and the target gene encoding Arg28-Ile160 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
ReactivityHuman
Protein SequenceRPPQFTRAQW FAIQHISLNP PRCTIAMRAI NNYRWRCKNQ NTFLRTTFAN VVNVCGNQSI RCPHNRTLNN CHRSRFRVPL LHCDLINPGA QNISNCRYAD RPGRRFYVVA CDNRDPRDSP RYPVVPVHLD TTIVDHHHHH H
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ECP; RNS3; Eosinophil cationic protein; ECP; Ribonuclease 3; RNase 3

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human ECP protein (orb392392)

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Available Sizes

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10 μg
$ 350.00
50 μg
$ 910.00
500 μg
$ 3,540.00
DispatchUsually dispatched within 5-10 working days
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