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Catalog Number | orb392392 |
---|---|
Category | Proteins |
Description | Recombinant human ECP protein is produced by our Mammalian expression system and the target gene encoding Arg28-Ile160 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | RNASE3 |
Entrez | 6037 |
UniProt ID | P12724 |
Protein Sequence | RPPQFTRAQW FAIQHISLNP PRCTIAMRAI NNYRWRCKNQ NTFLRTTFAN VVNVCGNQSI RCPHNRTLNN CHRSRFRVPL LHCDLINPGA QNISNCRYAD RPGRRFYVVA CDNRDPRDSP RYPVVPVHLD TTIVDHHHHH H |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5. |
Alternative names | ECP; RNS3; Eosinophil cationic protein; ECP; Ribon Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
67 kDa | |
E.coli |
Human | |
1.56 ng/mL-100 ng/mL | |
0.39 ng/mL |
Human | |
39.06-2500 pg/mL | |
13 pg/mL |
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