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Catalog Number | orb391568 |
---|---|
Category | Proteins |
Description | Recombinant human CTSB protein is produced by our mammalian expression system in human cells. The target protein is expressed with full length sequence of Human Cathepsin B fused with a polyhistidine tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | CTSB |
Entrez | 1508 |
UniProt ID | P07858 |
Protein Sequence | RSRPSFHPVS DELVNYVNKR NTTWQAGHNF YNVDMGYLKR LCGTFLGGPK PPQRVMFTED LKLPASFDAR EQWPQCPTIK EIRDQGSCGS CWAFGAVEAI SDRICIHTNA HVSVEVSAED LLTCCGSMCG DGCNGGYPAE AWNFWTRKGL VSGGLYESHV GCRPYSIPPC EHHVNGSRPP CTGEGDTPKC SKICEPGYSP TYKQDKHYGY NSYSVSNSEK DIMAEIYKNG PVEGAFSVYS DFLLYKSGVY QHVTGEMMGG HAIRILGWGV ENGTPYWLVA NSWNTDWGDN GFFKILRGQD HCGIESEVVA GIPRTDQYWE KIVDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of PBS, pH 7.4. |
Alternative names | CPSB; Cathepsin B; APP secretase; APPS; Cathepsin Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
36.7 kDa & 29.5 kDa | |
Human Cathepsin B, His Tag (orb257382) is expressed from human 293 cells (HEK293). It contains AA Arg 18 - Ile 339 (Accession # P07858-1 (L26V)). |
Greater than 90% as determined by SDS-PAGE. | |
54.6 kDa | |
E.coli |
Human | |
0.312 ng/mL-20 ng/mL | |
0.078 ng/mL |
Greater than 90% as determined by SDS-PAGE. | |
27.7 kDa | |
E.coli |
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