Human CTGF / CCN2 Protein

• Catalog Number: orb867319
• Category: Proteins
• Description: Connective Tissue Growth Factor (CTGF), also known as CCN2, is a member of the CCN (CCN1-6) family of modular matricellular proteins. Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain. CTGF promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Analysis of CCN2 function in vivo has focused primarily on its key role as a mediator of excess ECM synthesis in multiple fibrotic diseases.
• Reactivity: Human
• Tag: N-Fc
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 61.9 kDa
• Target: CTGF
• Source: Human CTGF, Fc Tag (orb867319) is expressed from human 293 cells (HEK293). It contains AA Gln27-Ala349 (Accession # Q5M8T4-1 ).
• Expression System: HEK293
• Biological Activity: Immobilized Human CTGF, Fc Tag at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.8-6 ng/mL (QC tested).
• Expression Region: Gln27-Ala349
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001892.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
• Alternative names: CCN2,NOV2,HCS24,IGFBP8,IBP-8,IGFBP-8,IGF-binding p
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 61.9 kDa. The protein migrates as 48-53 kDa and 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human CTGF, Fc Tag (Cat. No. orb867319) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.8-6 ng/mL (QC tested).