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Catalog Number | orb391551 |
---|---|
Category | Proteins |
Description | Recombinant human CST5 protein is produced by our Mammalian expression system and the target gene encoding Gly21-Val142 is expressed with a 6His tag at the C-terminus. |
Target | CST5 |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM MES, 150mM NaCl, pH 5.5 |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM MES, 150mM NaCl, pH 5.5 |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Protein Sequence | GSASAQSRTL AGGIHATDLN DKSVQCALDF AISEYNKVIN KDEYYSRPLQ VMAAYQQIVG GVNYYFNVKF GRTTCTKSQP NLDNCPFNDQ PKLKEEEFCS FQINEVPWED KISILNYKCR KVVDHHHHHH |
UniProt ID | P28325 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | Human cells |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | Cystatin-D; Cystatin-5 |
Note | For research use only |
Entrez | 1473 |
WB | |
Human | |
Mouse | |
Polyclonal | |
Unconjugated |
> 90% as determined by SDS-PAGE. | |
16.17 kDa |
> 95%, determined by SDS-PAGE | |
This protein contains the pro form of human Cystatin D (NP_001891.2) (Met 1-Val 142) with a C-terminal polyhistidine tag was expressed from HEK293 Cells. |
> 95%, determined by SDS-PAGE | |
This protein contains the pro form of human Cystatin D (NP_001891.2) (Met 1-Val 142) with a C-terminal polyhistidine tag was expressed from HEK293 Cells. |