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Catalog Number | orb391530 |
---|---|
Category | Proteins |
Description | Recombinant human CRTAM protein is produced by our mammalian expression system and the target gene encoding Ser18-Ser286 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CRTAM |
Entrez | 56253 |
UniProt ID | O95727 |
Protein Sequence | SLTNHTETIT VEEGQTLTLK CVTSLRKNSS LQWLTPSGFT IFLNEYPVLK NSKYQLLHHS ANQLSITVPN VTLQDEGVYK CLHYSDSVST KEVKVIVLAT PFKPILEASV IRKQNGEEHV VLMCSTMRSK PPPQITWLLG NSMEVSGGTL HEFETDGKKC NTTSTLIIHT YGKNSTVDCI IRHRGLQGRK LVAPFRFEDL VTDEETASDA LERNSLSSQD PQQPTSTVSV TEDSSTSEID KEEKEQTTQD PDLTTEANPQ YLGLARKKSV DHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | Cytotoxic and regulatory T-cell molecule; Class-I Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 30.8 kDa after removal of the signal peptide. The apparent molecular mass of CRTAM-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
90% | |
31.9 kDa | |
Human CRTAM, His Tag (orb612121) is expressed from human 293 cells (HEK293). It contains AA Ser 18 - Gly 287 (Accession # O95727-1). |
≥90% as determined by SDS-PAGE | |
This protein contains the human CRTAM(Met1-Ser286) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
≥90% as determined by SDS-PAGE | |
This protein contains the human CRTAM(Ser18-Ser286) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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