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Human CPE protein

SKU: orb391515

Description

Recombinant human CPE protein is produced by our mammalian expression system and the target gene encoding Arg42-Ser453 is expressed with a 6His tag at the C-terminus.

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
ReactivityHuman
Protein SequenceRLQQEDGISF EYHRYPELRE ALVSVWLQCT AISRIYTVGR SFEGRELLVI ELSDNPGVHE PGEPEFKYIG NMHGNEAVGR ELLIFLAQYL CNEYQKGNET IVNLIHSTRI HIMPSLNPDG FEKAASQPGE LKDWFVGRSN AQGIDLNRNF PDLDRIVYVN EKEGGPNNHL LKNMKKIVDQ NTKLAPETKA VIHWIMDIPF VLSANLHGGD LVANYPYDET RSGSAHEYSS SPDDAIFQSL ARAYSSFNPA MSDPNRPPCR KNDDDSSFVD GTTNGGAWYS VPGGMQDFNY LSSNCFEITV ELSCEKFPPE ETLKTYWEDN KNSLISYLEQ IHRGVKGFVR DLQGNPIANA TISVEGIDHD VTSAKDGDYW RLLIPGNYKL TASAPGYLAI TKKVAVPYSP AAGVDFELES FSVDHHHHHH
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM ZnCl2, 10% Glycerol, pH 7.5.
DisclaimerFor research use only

Alternative Names

Carboxypeptidase E; CPE; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human CPE protein (orb391515)

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Available Sizes

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10 μg
$ 340.00
50 μg
$ 910.00
500 μg
$ 3,390.00
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