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Human CPA1 protein

SKU: orb391508

Description

Recombinant human CPA1 protein is produced by our Mammalian expression system and the target gene encoding Lys17-Tyr419 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
ReactivityHuman
Protein SequenceKEDFVGHQVL RISVADEAQV QKVKELEDLE HLQLDFWRGP AHPGSPIDVR VPFPSIQAVK IFLESHGISY ETMIEDVQSL LDEEQEQMFA FRSRARSTDT FNYATYHTLE EIYDFLDLLV AENPHLVSKI QIGNTYEGRP IYVLKFSTGG SKRPAIWIDT GIHSREWVTQ ASGVWFAKKI TQDYGQDAAF TAILDTLDIF LEIVTNPDGF AFTHSTNRMW RKTRSHTAGS LCIGVDPNRN WDAGFGLSGA SSNPCSETYR GKFANSEVEV KSIVDFVKDH GNIKAFISIH SYSQLLMYPY GYKTEPVPDQ DELDQLSKAA VTALASLYGT KFNYGSIIKA IYQASGSTID WTYSQGIKYS FTFELRDTGR YGFLLPASQI IPTAKETWLA LLTIMEHTLN HPYVDHHHHH H
PurityGreater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.
DisclaimerFor research use only

Alternative Names

CPA; Carboxypeptidase A1

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human CPA1 protein (orb391508)

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Available Sizes

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10 μg
$ 350.00
50 μg
$ 910.00
500 μg
$ 3,540.00
DispatchUsually dispatched within 5-10 working days
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