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Catalog Number | orb424787 |
---|---|
Category | Proteins |
Description | Recombinant of human CLDN4 protein |
Form/Appearance | Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | Filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4. |
Purity | Greater than 90.0% as determined by SDS-PAGE. |
Protein Sequence | MKHHHHHHAS MWRVTAFIGS NIVTSQTIWE GLWMNCVVQS TGQMQCKVYD SLLALPQDLQ AAR LEVLFQG PTAHNIIQDF YNPLVASGQK REM MWRVTAFIGS NIVTSQTIWE GLWMNCVVQS TGQMQCKVYD SLLALPQDLQ AAR LEVLFQG PTAHNIIQDF YNPLVASGQK REM |
Application notes | Recombinant & Natural Proteins |
Source | Escherichia Coli |
Solubility (25°C) | It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
Storage | Stability: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C |
Alternative names | Claudin 4, CPE-R, CPE-receptor, Clostridium perfri Read more... |
Note | For research use only |
The human full length CLDN4 protein has a MW of 22.1 kDa | |
Mammalian |
FC, WB | |
Human | |
Mouse | |
Polyclonal | |
Unconjugated |
Greater than 90% as determined by SDS-PAGE. | |
28.1 kDa | |
in vitro E.coli expression system |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 27.7 kDa after removal of the signal peptide. The apparent molecular mass of CLDN4(145-157)-mFc is approximately 25-35 kDa due to glycosylation. | |
Mammalian |