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Catalog Number | orb865262 |
---|---|
Category | Proteins |
Description | Recombinant Human Claudin-18.2(CLDN18.2)-VLPs (Active) |
Tag | C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions) |
Form/Appearance | Lyophilized powder |
Buffer/Preservatives | Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4 |
Protein Sequence | MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV |
Protein Length | Full Length of Isoform A2 |
UniProt ID | P56856 |
MW | 29.1 kDa |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing. |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Source | Mammalian cell |
Biological Origin | Homo sapiens (Human) |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized human CLDN18.2 at 5 μg/ml can bind anti-CLDN18.2 recombinant Monoclonal Antibody (orb783578), the the EC50 is 5.225-9.256 ng/ml |
Expression Region | 1-261aa |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Alternative names | CLDN18.2 |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Western Blot is detected by Mouse anti-6*His monoclonal antibody.The three bands respectively correspond to monomer, Homodimer, Homotrimer
Measured by its binding ability in a functional ELISA. Immobilized human CLDN18.2 at 5 μg/ml can bind anti-CLDN18.2 recombinant Monoclonal Antibody, the the EC50 is 5.225-9.256 ng/ml.
The presence of VLP-like structures was confirmed by TEM
Western Blot is detected by Anti-Claudin 18 Antibody.The two bands respectively correspond to monomer, Homodimer
The human full length CLDN18.2 Protein has a MW of 27.5 kDa | |
Mammalian |
The human full length CLDN18.2 fusion protein has a MW of 54.2 kDa | |
Mammalian |
The Human Claudin 18.2 Protein has a predicted MW of 29 kDa.Due to PTM, the actual MW on SDS-PAGE gel is around 50kDa. | |
Mammalian |
The human full length CLDN18.2 Protein has a MW of 27.5 kDa | |
Mammalian |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 31.9 kDa after removal of the signal peptide. | |
Mammalian |