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Catalog Number | orb257343 |
---|---|
Category | Proteins |
Description | Human CHI3L1, His Tag is expressed from human 293 cells (HEK293). It contains AA Tyr 22 - Thr 383 (Accession # AAH38354). |
Target | Chitinase 3-like 1 |
Conjugation | Unconjugated |
Tag | C-6×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | AAH38354 |
UniProt ID | P36222 |
MW | 41.2 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 41.2 kDa. The protein migrates as 40-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Tyr 22 - Thr 383 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAH38354 |
SDS-PAGE analysis of Human Chitinase 3-like 1 protein
> 95%, determined by SDS-PAGE | |
This protein contains the human CHI3L1 (NP_0012672) (Met 1-Thr 383) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
> 95%, determined by SDS-PAGE | |
This protein contains the human CHI3L1 (NP_0012672) (Met 1-Thr 383) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
CLIA, EIA, LF | |
> 95% as determined by SDS-PAGE. | |
42.2 kDa | |
HEK293 |
> 95% by SDS-PAGE. | |
Recombinant Human Chitinase-3-like protein 1/CHI3L1 Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Tyr22-Thr383) of human Chitinase-3-like protein 1/CHI3L1 (Accession #P36222) fused with a 6��His Tag at the C-terminus. |