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Human CGREF1 protein

SKU: orb391477

Description

Recombinant human CGREF1 protein is produced by our mammalian expression system and the target gene encoding Ala20-Ile301 is expressed with a 6His tag at the C-terminus.

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
ReactivityHuman
Protein SequenceAPKDGVTRPD SEVQHQLLPN PFQPGQEQLG LLQSYLKGLG RTEVQLEHLS REQVLLYLFA LHDYDQSGQL DGLELLSMLT AALAPGAANS PTTNPVILIV DKVLETQDLN GDGLMTPAEL INFPGVALRH VEPGEPLAPS PQEPQAVGRQ SLLAKSPLRQ ETQEAPGPRE EAKGQVEARR ESLDPVQEPG GQAEADGDVP GPRGEAEGQA EAKGDAPGPR GEAGGQAEAE GDAPGPRGEA GGQAEARENG EEAKELPGET LESKNTQNDF EVHIVQVEND EIVDHHHHHH
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM CaCl2, pH 7.5.
DisclaimerFor research use only

Alternative Names

CGR11; Cell growth regulator with EF hand domain protein 1; Cell growth regulatory gene 11 protein; Hydrophobestin

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human CGREF1 protein (orb391477)

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Available Sizes

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10 μg
$ 270.00
50 μg
$ 650.00
500 μg
$ 3,100.00
DispatchUsually dispatched within 5-10 working days
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