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Catalog Number | orb391472 |
---|---|
Category | Proteins |
Description | Recombinant human CFH protein is produced by our mammalian expression system and the target gene encoding Glu19-Leu449 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of PBS, 20% Glycerol, 5% Trehalose, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CFH |
Entrez | 3075 |
UniProt ID | P08603 |
Protein Sequence | EDCNELPPRR NTEILTGSWS DQTYPEGTQA IYKCRPGYRS LGNVIMVCRK GEWVALNPLR KCQKRPCGHP GDTPFGTFTL TGGNVFEYGV KAVYTCNEGY QLLGEINYRE CDTDGWTNDI PICEVVKCLP VTAPENGKIV SSAMEPDREY HFGQAVRFVC NSGYKIEGDE EMHCSDDGFW SKEKPKCVEI SCKSPDVING SPISQKIIYK ENERFQYKCN MGYEYSERGD AVCTESGWRP LPSCEEKSCD NPYIPNGDYS PLRIKHRTGD EITYQCRNGF YPATRGNTAK CTSTGWIPAP RCTLKPCDYP DIKHGGLYHE NMRRPYFPVA VGKYYSYYCD EHFETPSGSY WDHIHCTQDG WSPAVPCLRK CYFPYLENGY NQNYGRKFVQ GKSIDVACHP GYALPKAQTT VTCMENGWSP TPRCIRVSFT LVDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of PBS, 20% Glycerol, 5% Trehalose, pH 7.4. |
Alternative names | HF; HF1; HF2; Complement factor H; H factor 1 Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Human | |
31.2 ng/mL-2000 ng/mL | |
7.8 ng/ml |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 138.4 kDa after removal of the signal peptide. The apparent molecular mass of CFH-His is approximately 130-250 kDa due to glycosylation. | |
Mammalian |
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