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Human CFH protein

SKU: orb391472

Description

Recombinant human CFH protein is produced by our mammalian expression system and the target gene encoding Glu19-Leu449 is expressed with a 6His tag at the C-terminus.

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
ReactivityHuman
Protein SequenceEDCNELPPRR NTEILTGSWS DQTYPEGTQA IYKCRPGYRS LGNVIMVCRK GEWVALNPLR KCQKRPCGHP GDTPFGTFTL TGGNVFEYGV KAVYTCNEGY QLLGEINYRE CDTDGWTNDI PICEVVKCLP VTAPENGKIV SSAMEPDREY HFGQAVRFVC NSGYKIEGDE EMHCSDDGFW SKEKPKCVEI SCKSPDVING SPISQKIIYK ENERFQYKCN MGYEYSERGD AVCTESGWRP LPSCEEKSCD NPYIPNGDYS PLRIKHRTGD EITYQCRNGF YPATRGNTAK CTSTGWIPAP RCTLKPCDYP DIKHGGLYHE NMRRPYFPVA VGKYYSYYCD EHFETPSGSY WDHIHCTQDG WSPAVPCLRK CYFPYLENGY NQNYGRKFVQ GKSIDVACHP GYALPKAQTT VTCMENGWSP TPRCIRVSFT LVDHHHHHH
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of PBS, 20% Glycerol, 5% Trehalose, pH 7.4.
DisclaimerFor research use only

Alternative Names

HF; HF1; HF2; Complement factor H; H factor 1

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human CFH protein (orb391472)

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Available Sizes

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10 μg
$ 340.00
50 μg
$ 910.00
500 μg
$ 3,390.00
DispatchUsually dispatched within 5-10 working days
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