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Human CFB protein

SKU: orb391470

Description

Recombinant human CFB protein is produced by our mammalian expression system and the target gene encoding Thr26-Leu764 is expressed with a 6His tag at the C-terminus.

Research Area

Immunology & Inflammation

Images & Validation

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Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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SourceHuman cells
TargetCFB
ReactivityHuman
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceTPWSLAWPQG SCSLEGVEIK GGSFRLLQEG QALEYVCPSG FYPYPVQTRT CRSTGSWSTL KTQDQKTVRK AECRAIHCPR PHDFENGEYW PRSPYYNVSD EISFHCYDGY TLRGSANRTC QVNGRWSGQT AICDNGAGYC SNPGIPIGTR KVGSQYRLED SVTYHCSRGL TLRGSQRRTC QEGGSWSGTE PSCQDSFMYD TPQEVAEAFL SSLTETIEGV DAEDGHGPGE QQKRKIVLDP SGSMNIYLVL DGSDSIGASN FTGAKKCLVN LIEKVASYGV KPRYGLVTYA TYPKIWVKVS EADSSNADWV TKQLNEINYE DHKLKSGTNT KKALQAVYSM MSWPDDVPPE GWNRTRHVII LMTDGLHNMG GDPITVIDEI RDLLYIGKDR KNPREDYLDV YVFGVGPLVN QVNINALASK KDNEQHVFKV KDMENLEDVF YQMIDESQSL SLCGMVWEHR KGTDYHKQPW QAKISVIRPS KGHESCMGAV VSEYFVLTAA HCFTVDDKEH SIKVSVGGEK RDLEIEVVLF HPNYNINGKK EAGIPEFYDY DVALIKLKNK LKYGQTIRPI CLPCTEGTTR ALRLPPTTTC QQQKEELLPA QDIKALFVSE EEKKLTRKEV YIKNGDKKGS CERDAQYAPG YDKVKDISEV VTPRFLCTGG VSPYADPNTC RGDSGGPLIV HKRSRFIQVG VISWGVVDVC KNQKRQKQVP AHARDFHINL FQVLPWLKEK LQDEDLGFLV DHHHHHH

Storage & Handling

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StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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BF; BFD; Complement factor B; C3/C5 convertase; Glycine-rich beta glycoprotein; GBG; PBF2; Properdin factor B

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UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

10 μg
$ 300.00
50 μg
$ 680.00
500 μg
$ 3,230.00
DispatchUsually dispatched within 5-10 working days
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