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Human CELA3A protein

SKU: orb391465

Description

Recombinant human CELA3A protein is produced by our mammalian expression system and the target gene encoding Ser16-His270 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

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Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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SourceHuman cells
TargetCELA3A
ReactivityHuman
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceSGYGPPSSHS SSRVVHGEDA VPYSWPWQVS LQYEKSGSFY HTCGGSLIAP DWVVTAGHCI SRDLTYQVVL GEYNLAVKEG PEQVIPINSE ELFVHPLWNR SCVACGNDIA LIKLSRSAQL GDAVQLASLP PAGDILPNKT PCYITGWGRL YTNGPLPDKL QQARLPVVDY KHCSRWNWWG STVKKTMVCA GGYIRSGCNG DSGGPLNCPT EDGGWQVHGV TSFVSGFGCN FIWKPTVFTR VSAFIDWIEE TIASHVDHHH HHH

Storage & Handling

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StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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ELA3; ELA3A; Chymotrypsin-like elastase family member 3A; Elastase IIIA; Elastase-3A; Protease E

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UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

10 μg
$ 370.00
50 μg
$ 960.00
500 μg
$ 3,690.00
DispatchUsually dispatched within 5-10 working days
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