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Catalog Number | orb391448 |
---|---|
Category | Proteins |
Description | Recombinant human CDK2 protein is produced by our E.coli expression system and the target gene encoding Met1-Leu298 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 200mM NaCl, 1mM DTT, 40% Glycerol, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CDK2 |
Entrez | 1017 |
UniProt ID | P24941 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINTEGA IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY STAVDIWSLG CIFAEMVTRR ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF PKWARQDFSK VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRL |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 200mM NaCl, 1mM DTT, 40% Glycerol, pH 8.0. |
Alternative names | CDKN2; Cyclin-dependent kinase 2; Cell division pr Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 85% as determined by SDS-PAGE. | |
25.8 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
37.9 kDa | |
E.coli |
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