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Catalog Number | orb391425 |
---|---|
Category | Proteins |
Description | Recombinant human CD72 protein is produced by our E. coli expression system and the target gene encoding Arg117-Cys226 is expressed with a Trx, 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CD72 |
Entrez | 971 |
UniProt ID | P21854 |
Protein Sequence | MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG SGMKETAAAK FERQHMDSPD LGTDDDDKAM ADIGSMRYLQ VSQQLQQTNR VLEVTNSSLR QQLRLKITQL GQSAEDLQGS RRELAQSQEA LQVEQRAHQA AEGQLQACQA DRQKTKETLQ SEEQQRRALE QKLSNMENRL KPFFTC |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | B-cell differentiation antigen CD72; Lyb-2; CD72 Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
105.4 kDa | |
Human SEMA4D, Fc Tag (orb257252) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Arg 734 (Accession # AAH54500). |
Unconjugated | |
95% | |
54.2 kDa | |
Human CD72, Fc Tag (orb257261) is expressed from human 293 cells (HEK293). It contains AA Arg 117 - Asp 359 (Accession # AAH30227). |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 54.2 kDa after removal of the signal peptide. The apparent molecular mass of hFc-CD72 is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
90% | |
55.0 kDa | |
Human CD72, Mouse IgG2a Fc Tag (orb1087564) is expressed from human 293 cells (HEK293). It contains AA Arg 117 - Asp 359 (Accession # P21855-1). |
≥90% as determined by SDS-PAGE | |
This protein contains the human CD72(Arg117-Asp359) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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