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| Catalog Number | orb391418 |
|---|---|
| Category | Proteins |
| Description | Recombinant human CD47 protein is produced by our mammalian expression system and the target gene encoding Gln19-Pro139 is expressed with a Fc tag at the C-terminus. |
| Reactivity | Human |
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PVDDIEGRMD EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK |
| UniProt ID | Q08722 |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Source | Human cells |
| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
| Alternative names | MER6; Leukocyte surface antigen CD47; Antigenic su Read more... |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 961 |
| Expiration Date | 6 months from date of receipt. |
Human | |
0.32-20 ng/mL | |
0.111 ng/mL |
Human | |
0.16-10ng/mL | |
0.1 ng/mL |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 60 kDa after removal of the signal peptide. | |
Mammalian |
Biotin | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 16.4 KDa. Observed: 30-40 KDa, reducing conditions |
Unconjugated | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 40.3 KDa. Observed: 48-65 KDa, reducing conditions |
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