Human CD44 Protein

• Catalog Number: orb257269
• Category: Proteins
• Description: CD44 antigen is a cell-surface glycoprotein involved in cell–cell interactions, cell adhesion and migration. CD44 is expressed in a large number of mammalian cell types. CD44 is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). CD44 function is controlled by its posttranslational modifications. One critical modification involves discrete sialofucosylations rendering the selectin-binding glycoform of CD44 called HCELL (for Hematopoietic Cell E-selectin/L-selectin Ligand). CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms.
• Reactivity: Human
• Tag: C-9×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 23.3 kDa
• Target: CD44
• Protein Sequence: AAH04372
• Source: Human CD44, His Tag (orb257269) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Pro 220 (Accession # AAH04372).
• Expression System: HEK293
• Expression Region: Gln 21 - Pro 220
• Endotoxins: 1.0 EU per μg
• NCBI: AAH04372
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: CD44,CDW44,CSPG8,ECMR-III,HCELL,HUTCH-I,IN,LHR,MC5
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.3 kDa. The protein migrates as 32-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human CD44 protein

Immobilized Human CD44, His Tag at 5 ug/mL (100 μL/well) can bind Hyaluronan biotin sodium salt with a linear range of 39-156 ng/mL (Routinely tested).