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Human CD3E protein
Description
Research Area
Images & Validation
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| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human cells |
|---|---|
| Reactivity | Human |
| Protein Sequence | DGNEEMGGIT QTPYKVSISG TTVILTCPQY PGSEILWQHN DKNIGGDEDD KNIGSDEDHL SLKEFSELEQ SGYYVCYPRG SKPEDANFYL YLRARVCENC MEMDVDHHHH HH |
| Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−CD3e Antibody [orb665922]
IF, IHC, IP, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
30 μl, 50 μl, 100 μl, 200 μlHuman CD3D Protein, His Tag and Human CD3E Protein, hFc Tag [orb1743385]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 10.4 and 37.9 kDa after removal of the signal peptide. The apparent molecular mass of CD3D-His and CD3E-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
100 μg, 50 μg, 10 μgHuman CD3E & CD3G Heterodimer Protein, Fc-His Tag & Fc-Flag Tag [orb348813]
Unconjugated
95%
39.2 kDa (CD3E) & 38.6 kDa (CD3G)
50 μg, 1 mg

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Protocol Information
Human CD3E protein (orb391409)
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