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Human CD3E protein
Description
Research Area
Images & Validation
−
| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human cells |
|---|---|
| Target | CD3E |
| Reactivity | Human |
| Protein Sequence | DGNEEMGGIT QTPYKVSISG TTVILTCPQY PGSEILWQHN DKNIGGDEDD KNIGSDEDHL SLKEFSELEQ SGYYVCYPRG SKPEDANFYL YLRARVCENC MEMDVDHHHH HH |
| Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 14.6&16.0 KDa. Observed: 20&(22-38) KDa, reducing conditions
1 mg, 500 μg, 50 μg, 10 μgRecombinant Cynomolgus CD3D&CD3E Heterodimer Protein [orb2993714]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 37&38.3 KDa. Observed: 40-60 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human CD3E&CD3G Heterodimer Protein [orb2993735]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 39.2&38.1 KDa. Observed: 40-60 KDa, reducing conditions
1 mg, 10 μg, 50 μg, 500 μgRecombinant Human CD3E Protein (Biotin) [orb2993856]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 40.5 KDa. Observed: 45-60 KDa, reducing conditions
20 μg, 100 μg

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Protocol Information
Human CD3E protein (orb391409)
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