Human CD3E & CD3G Heterodimer Protein, Fc-His Tag & Fc-Flag Tag

• Catalog Number: orb348813
• Category: Proteins
• Description: Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1 (CD3E) & P09693-1 (CD3G)).
• Target: CD3E & CD3G
• Conjugation: Unconjugated
• Tag: C-Fc & C-6×His | C-Fc & C-Flag
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• Protein Sequence: NP_000724.1 (CD3E) & AAI13831.1 (CD3G)
• UniProt ID: P07766, P09693
• MW: 39.2 kDa (CD3E) & 38.6 kDa (CD3G)
• Application notes: Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Human
• Expression Region: Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: NP_000724.1, AAI13831.1, CD3

SDS-PAGE analysis of Human CD3 epsilon & CD3 gamma Heterodimer protein

Immobilized OKT3 MAb at 10ug/mL (100 uL/well) can bind Human CD3E & CD3G with a linear range of 0.3-5 ug/mL.