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Catalog Number | orb391400 |
---|---|
Category | Proteins |
Description | Recombinant human CD300A protein is produced by our mammalian expression system and the target gene encoding Leu18-Gln178 is expressed with a Fc, 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 5% Trehalose, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CD300A |
Entrez | 11314 |
UniProt ID | Q9UGN4 |
Protein Sequence | LSKCRTVAGP VGGSLSVQCP YEKEHRTLNK YWCRPPQIFL CDKIVETKGS AGKRNGRVSI RDSPANLSFT VTLENLTEED AGTYWCGVDT PWLRDFHDPV VEVEVSVFPA STSMTPASIT AAKTSTITTA FPPVSSTTLF AVGATHSASI QEETEEVVNS QVDDIEGRMD EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 5% Trehalose, pH 8.0. |
Alternative names | CMRF35H; IGSF12; CMRF35-like molecule 8; CLM-8; CD Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
18.67 kDa | |
Human CD300c, His Tag (orb257316) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Arg 183 (Accession # AAH22279). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
Greater than 85% as determined by SDS-PAGE. | |
49.1 | |
E.coli |
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