Human CD30 Ligand Protein, mFc-His Tag

• Catalog Number: orb689400
• Category: Proteins
• Description: Recombinant human CD30 Ligand Protein with N-terminal mouse Fc and C-terminal 6×His tag
• Reactivity: Human
• Tag: N-Mouse Fc and C-6×His Tag
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• MW: The protein has a predicted molecular mass of 48.0 kDa after removal of the signal peptide.The apparent molecular mass of mFc-CD30 Ligand-His is approximately 53-70 kDa due to glycosylation.
• Target: CD30 Ligand
• UniProt ID: P32971
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Alternative names: CD30-L;CD153;TNFSF8;CD30L;CD30LG;CD153 antigen;CD3
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human B7H2 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

HEK293 cell line transfected with irrelevant protein (B) and human ICOS (A) were surface stained with Human B7-H2, mFc-His tagged protein (orb689390) 1 μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human B7-H2 Protein, mFc-His Tag (orb689390) can bind Anti-B7-H2 Rabbit mAb in a linear range of 3.2-80 ng/ml.