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| Catalog Number | orb1152447 |
|---|---|
| Category | Proteins |
| Description | Human CD171, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 1120 (Accession # P32004-1). |
| Target | CD171 |
| Conjugation | Unconjugated |
| Tag | C-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | 50 mM Tris, 150 mM NaCl, pH 8.0 |
| Purity | 95% |
| UniProt ID | P32004 |
| MW | 125.1 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 125.1 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 0.2 EU per μg |
| Expression Region | Ile 20 - Glu 1120 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Disease Biomarkers |
| Note | For research use only |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 124.0 kDa after removal of the signal peptide.The apparent molecular mass of CD171-His is approximately 130-250 kDa due to glycosylation. | |
Mammalian |
> 85% as determined by SDS-PAGE |
>90% as determined by SDS-PAGE. | |
126.32 kDa |
>90% as determined by SDS-PAGE. | |
25.53 kDa |
>85%, determined by SDS-PAGE | |
This protein contains human CD171 (NP_0004161) (Met 1-Glu 1120) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
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