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Catalog Number | orb257270 |
---|---|
Category | Proteins |
Description | Cluster of differentiation 14 (CD14), is a cell surface glycoprotein, and is a is a component of the innate immune system. CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. CD14 exists in two forms. Either it is anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa). CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). CD14 has been proposed to be involved in various biological processes, including transportation of other lipids, cell-cell interaction during different immune responses, as well as recognition of apoptotic cells. Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. CD14+ cells are monocytes that can differentiate into a host of different cells. CD14 has been shown to interact with Lipopolysaccharide-binding protein. |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 98% |
Conjugation | Unconjugated |
MW | 35.9 kDa |
Target | CD14 |
Protein Sequence | AAH10507 |
Source | Human CD14, His Tag (orb257270) is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Met 344 (Accession # AAH10507). |
Expression System | HEK293 |
Expression Region | Thr 20 - Met 344 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH10507 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | CD14 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.9 kDa. The protein migrates as 40-53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human CD14 protein
Unconjugated | |
95% | |
52.8 kDa | |
Human Lipopolysaccharide, His Tag (orb257660) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Val 481 (Accession # P18428-1 (I465F)). |
Unconjugated | |
95% | |
62.2 kDa | |
Human CD14, Fc Tag (orb257273) is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Cys 352 (Accession # P08571-1). |
Greater than 90% as determined by SDS-PAGE. | |
62.2 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
39.2 kDa | |
Mammalian cell |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
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