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Catalog Number | orb391370 |
---|---|
Category | Proteins |
Description | Recombinant human CCL27 protein is produced by our E. coli. It is a single non-glycosylated polypeptide chain containing 88 amino acids with a molecular mass of 10.1 kD. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 500mM NaCl, pH 7.5 |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | CCL27 |
Entrez | 10850 |
UniProt ID | Q9Y4X3 |
Protein Sequence | FLLPPSTACC TQLYRKPLSD KLLRKVIQVE LQEADGDCHL QAFVLHLAQR SICIHPQNPS LSQWFEHQER KLHGTLPKLN FGMLRKMG |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 500mM NaCl, pH 7.5 |
Alternative names | ILC; SCYA27; C-C motif chemokine 27; CC chemokine Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 96% as determined by SDS-PAGE and HPLC. | |
10.1 kDa | |
E.Coli |
Unconjugated | |
90% | |
12.2 kDa | |
Human CCL27 Protein, His Tag (orb1786149) is expressed from E. coli cells. It contains AA Phe 25 - Gly 112 (Accession # Q9Y4X3). |
Greater than 95% as determined by reducing SDS-PAGE. | |
10.1 KDa | |
Mammalian |
98.00% | |
15 KDa (reducing condition) |