Human CASP10 protein

SKU: orb391355

Description

Recombinant human CASP10 protein is produced by our E. coli expression system and the target gene encoding Val220-Ile480 is expressed with a 6His tag at the C-terminus.

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Reactivity: Human
• Protein Sequence: MVKTFLEALP QESWQNKHAG SNGNRATNGA PSLVSRGMQG ASANTLNSET STKRAAVYRM NRNHRGLCVI VNNHSFTSLK DRQGTHKDAE ILSHVFQWLG FTVHIHNNVT KVEMEMVLQK QKCNPAHADG DCFVFCILTH GRFGAVYSSD EALIPIREIM SHFTALQCPR LAEKPKLFFI QACQGEEIQP SVSIEADALN PEQAPTSLQD SIPAEADFLL GLATVPGYVS FRHVEEGSWY IQSLCNHLKK LVPRHEDILS ILEHHHHHH
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid in a 0.2 μM filtered solution of 25mM HEPES, 10mM DTT, pH 7.5.
• Disclaimer: For research use only

Alternative Names

ALPS2; apoptotic protease Mch-4; CASP-10; CASPA; caspase-10; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-convert

Quick Database Links

UniProt

Q92851

NCBI Gene ID

843