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Human CA3 protein

SKU: orb391336

Description

Recombinant human CA3 protein is produced by our E. coli expression system and the target gene encoding Ala2-Lys260 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
ReactivityHuman
Protein SequenceAKEWGYASHN GPDHWHELFP NAKGENQSPI ELHTKDIRHD PSLQPWSVSY DGGSAKTILN NGKTCRVVFD DTYDRSMLRG GPLPGPYRLR QFHLHWGSSD DHGSEHTVDG VKYAAELHLV HWNPKYNTFK EALKQRDGIA VIGIFLKIGH ENGEFQIFLD ALDKIKTKGK EAPFTKFDPS CLFPACRDYW TYQGSFTTPP CEECIVWLLL KEPMTVSSDQ MAKLRSLLSS AENEPPVPLV SNWRPPQPIN NRVVRASFKL EHHHHHH
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of 25mM Tris-HCl, 150mM NaCl, pH 8.5.
DisclaimerFor research use only

Alternative Names

Carbonic anhydrase 3; Carbonate dehydratase III; Carbonic anhydrase III; CA-III

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human CA3 protein (orb391336)

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Available Sizes

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10 μg
$ 260.00
50 μg
$ 600.00
500 μg
$ 3,450.00
DispatchUsually dispatched within 5-10 working days
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