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Catalog Number | orb391336 |
---|---|
Category | Proteins |
Description | Recombinant human CA3 protein is produced by our E. coli expression system and the target gene encoding Ala2-Lys260 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 25mM Tris-HCl, 150mM NaCl, pH 8.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CA3 |
Entrez | 761 |
UniProt ID | P07451 |
Protein Sequence | AKEWGYASHN GPDHWHELFP NAKGENQSPI ELHTKDIRHD PSLQPWSVSY DGGSAKTILN NGKTCRVVFD DTYDRSMLRG GPLPGPYRLR QFHLHWGSSD DHGSEHTVDG VKYAAELHLV HWNPKYNTFK EALKQRDGIA VIGIFLKIGH ENGEFQIFLD ALDKIKTKGK EAPFTKFDPS CLFPACRDYW TYQGSFTTPP CEECIVWLLL KEPMTVSSDQ MAKLRSLLSS AENEPPVPLV SNWRPPQPIN NRVVRASFKL EHHHHHH |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 25mM Tris-HCl, 150mM NaCl, pH 8.5. |
Alternative names | Carbonic anhydrase 3; Carbonate dehydratase III; C Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Human | |
7.8 ng/mL-500 ng/mL | |
1.95 ng/mL |
≥90% as determined by SDS-PAGE | |
This protein contains the human CA3(Ala2-Lys260) was fused with the C-terminal His Tag and expressed in E. coli. |
ELISA, WB | |
Greater than 95% by SDS-PAGE gel analyses | |
31.3 KDa | |
E.Coli |
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